Search results for " Zymography"

showing 3 items of 3 documents

Zymographic detection and clinical correlations of MMP-2 and MMP-9 in breast cancer sera.

2004

Matrix metalloproteinases, in particular the gelatinases MMP-2 and MMP-9, have received great attention in recent years as putative tumour markers for clinical applications. The main reason for the observed interest is their easy detection in body fluids. Moreover, recent evidence has shown multiple functions of MMPs, rather than simply degrading ECM, which include the mobilisation of growth factors and processing of surface molecules. Several authors have reported increased levels of MMPs in a number of cancers, but clinical correlations in breast cancer are still fragmentary. Thus, the aim of the present research was to investigate the activity levels of circulating gelatinases in the ser…

OncologyCancer Researchmedicine.medical_specialtyGelatinasesmatrix metalloproteinaseReceptor ErbB-2gelatin zymographyMammary glandGelatinase ABreast NeoplasmsBiologyMatrix metalloproteinaseBreast cancerbreast cancerInternal medicineProgesterone receptormedicineBiomarkers TumorGelatinaseHumansSettore BIO/06 - Anatomia Comparata E CitologiaLymph nodeMolecular and Cellular Pathologymatrix metalloproteinasesclinic correlationsmedicine.diseasePrognosismedicine.anatomical_structureEndocrinologyOncologyMatrix Metalloproteinase 9Receptors EstrogenLymphatic MetastasisMatrix Metalloproteinase 2FemaleReceptors ProgesteroneBritish journal of cancer
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Zymographic analysis of circulating and tissue forms of colon carcinoma gelatinase A (MMP-2) and B (MMP-9) separated by mono- and two-dimensional ele…

2001

Gelatinase A (MMP-2) and gelatinase B (MMP-9) play a key role in the proteolytic cascade leading to ECM degradation during invasion and metastasis. The enzyme activity is regulated both at the intra- and extra-cellular level. Extracellular regulation is achieved mainly through the balance between proenzyme activation and inhibition, which appears to be altered in cancer patients. One of the mechanisms of MMP inhibition is the binding of the enzymes to appropriate tissue inhibitors (TIMP). In the recent literature, it has been suggested that MMP-2 and/or MMP-9 are indeed over-produced in many carcinomas, while the identity of the various enzymatic forms (latent, activated and enzyme/inhibito…

AdultGelatin ZymographyGelatinase AMatrix metalloproteinaseBiologyMetastasisExtracellularmedicineGelatinaseHumansElectrophoresis Gel Two-DimensionalMolecular Biologychemistry.chemical_classificationEnzyme Precursorsmedicine.diseaseMolecular biologyEnzyme assayEnzymeBiochemistrychemistryMatrix Metalloproteinase 9Colonic Neoplasmsbiology.proteinMatrix Metalloproteinase 2Electrophoresis Polyacrylamide GelDensitometryMatrix biology : journal of the International Society for Matrix Biology
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Manganese overload affects p38 MAPK phosphorylation and metalloproteinase activity during sea urchin embryonic development.

2014

Abstract In the marine environment, manganese represents a potential emerging contaminant, resulting from an increased production of manganese-containing compounds. In earlier reports we found that the exposure of Paracentrotus lividus sea urchin embryos to manganese produced phenotypes with no skeleton. In addition, manganese interfered with calcium uptake, perturbed extracellular signal-regulated kinase (ERK) signaling, affected the expression of skeletogenic genes, and caused an increase of the hsc70 and hsc60 protein levels. Here, we extended our studies focusing on the temporal activation of the p38 mitogen-activated protein kinase (p38 MAPK) and the proteolytic activity of metalloprot…

MAPK/ERK pathwayEmbryo NonmammalianAquatic ScienceBiologyMatrix metalloproteinaseOceanographyp38 Mitogen-Activated Protein KinasesParacentrotus lividusbiology.animalECM ERK Embryo-toxicity Immunoblotting MAPK MMPs Marine organisms' calcification Mn SDS-PAGE Zymography extracellular matrix extracellular signal-regulated kinase manganese metalloproteinases mitogen-activated protein kinase p38 MAPK sodium dodecyl sulfate-polyacrylamide gel electrophoresisAnimalsSettore BIO/06 - Anatomia Comparata E CitologiaPhosphorylationProtein kinase ASea urchinManganeseKinaseGeneral Medicinebiology.organism_classificationPollutionMatrix MetalloproteinasesBiochemistryMitogen-activated protein kinasebiology.proteinParacentrotusPhosphorylationWater Pollutants ChemicalMarine environmental research
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